刘俊杰 博士

副教授


2007-2011,四川大学,生命科学学院,学士

2011-2016,球盟会qmh网页版登录,生命科学学院,博士

2016-2018,劳伦斯-伯克利国家实验室--生物物理和生物成像中心,博士后

2018-2020,加州大学伯克利分校--细胞分子生物学系,博士后

2018-2020,美国生命科学研究基金(LSRF)研究员

2020至今  球盟会qmh网页版登录生命科学学院,助理教授、副教授

2020至今  清华-北大生命科学联合中心、北京生物结构前沿研究中心,研究员

 

●  研究兴趣、领域:


刘俊杰实验室采用多学科方法研究以下课题:

(1) 新型基因编辑工具的设计和开发

(2) RNA相关核酸酶机器的机制研究


●  代表性论文:


1. Liu ZX#, Zhang S#, Zhu HZ#, Chen ZH#, Yang Y#, Li LQ#, Lei Y, Liu Y, Li DY, Sun A, Li CP, Tan SQ, Wang GL, Shen JY, Jin S, Gao C, Liu JJ*. Hydrolytic endonucleolytic ribozyme (HYER) is programmable for sequence-specific DNA cleavage. Science, 2024 Feb 2; 383(6682): eadh4859.

2. Deng P#, Tan SQ#, Yang QY, Fu L, Wu Y, Zhu HZ, Sun L, Bao Z, Lin Y, Zhang QC, Wang H, Wang J*, Liu JJ*. Structural RNA components supervise the sequential DNA cleavage in R2 retrotransposon. Cell, 2023 Jun 22;186(13):2865-2879.

3. Li DY#, Li LQ#, Liu JJ*. Nucleases in gene-editing technologies: past and prologue. National Science Open, 2023 Jul 18;2(5): 20220067.

4.Hu Z#, Sun A#, Yang J, Naz G, Sun G, Li Z, Liu JJ*, Zhang S*, Zhang X*. Regulation of the CRISPR-Cas12a system by methylation and demethylation of guide RNA. Chemical Science, 2023,14(22):5945-5955.

5. Sun A#, Li CP#, Chen Z#, Zhang S#, Li DY, Yang Y, Li LQ, Zhao Y, Wang K, Li Z, Liu J, Liu S, Wang J*, Liu JJ*. The compact Casπ (Cas12l) ‘bracelet’ provides a unique structural platform for DNA manipulation. Cell Research, 2023 Mar;33(3), 229–244.

6. Tsuchida CA#, Zhang S#, Doost MS#, Zhao Y#, Wang J, O'Brien E, Fang H, Li CP, Li D, Hai ZY, Chuck J, Brötzmann J, Vartoumian A, Burstein D, Chen XW, Nogales E, Doudna JA*, Liu JJ*. Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activity. Mol Cell., 2022 Mar 17;82(6):1199-1209.

7. Yang M, Sun R, Deng P, Yang Y, Wang W, Liu JJ*, Chen C*. Nonspecific interactions between SpCas9 and dsDNA sites located downstream of the PAM mediate facilitated diffusion to accelerate target search. Chem Sci., 2021 Aug 20;12(38):12776-12784.

8. Liu JJ*, Wang HW*. Cryo-Electron Microscopy of Endogenous Yeast Exosomes. Methods in Molecular Biology, 2020;2062:401-415. (Book Chapter)

9. Liu TY#, Liu JJ#, Aditham AJ, Nogales E, Doudna JA*. Target preference of Type III-A CRISPR-Cas complexes at the transcription bubble. Nat Commun., 2019 Jul 5;10(1):3001.

10. Liu JJ#, Orlova N#, Oakes BL#, Ma E, Spinner HB, Baney KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brötzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E*, Doudna JA*. Author Correction: CasX enzymes comprise a distinct family of RNA-guided genome editors. Nature, 2019 Apr;568(7752):E8-E10.

11. Jiang F#, Liu JJ#, Osuna BA#, Xu M, Berry JD, Rauch BJ, Nogales E, Bondy-Denomy J*, Doudna JA*. Temperature-Responsive Competitive Inhibition of CRISPR-Cas9. Mol Cell., 2019 Feb 7;73(3):601-610.

12. Wright AV#, Liu JJ#, Knott GJ, Doxzen KW, Nogales E, Doudna JA*. Structures of the CRISPR genome integration complex. Science, 2017 Sep 15;357(6356):1113-1118.


#: Co-first author;    *: Corresponding author


 

●  联系方式:


电子邮件:junjiegogoliu(at)tsinghua.edu.cn (联系时请把(at)改成@)

实验室网站:http://gogolab.life.tsinghua.edu.cn

电话:+86-010-62770270 (lab)    +86-010-62770276 (office)

实验室通讯地址:北京市海淀区球盟会qmh网页版登录生物医学馆A401