李赛 博士

副教授


2018年-至今: 球盟会qmh网页版登录球盟会qmh网页版登录 助理教授、副教授

2018年-至今: 清华-北大生命联合中心 、北京生物结构前沿研究中心 研究员

2017-2018年:牛津大学结构生物学中心(STRUBI)Senior Research Associate                    

2013-2017年:牛津大学结构生物学中心(STRUBI)博士后

2009-2012年:哥廷根大学生物物理系 博士

2006-2008年:斯图加特大学物理系 硕士

2002-2006年:武汉大学应用物理系 学士

 

●  研究兴趣、领域:


1. 高分辨冷冻电镜断层成像(cryo-ET)方法。高分辨cryo-ET是使用冷冻电镜收集三维断层图像,再使用子断层图像平均法进行结构解析的跨尺度原位结构生物学方法。Cryo-ET不仅擅长解析动态组装的巨型分子机器结构,与结合光电联合成像(CLEM)、冷冻聚焦离子束(cryo-FIB)等技术结合,更可在细胞中进行成像和大分子结构原位解析,获得具有生理意义的原位结构。Cryo-ET技术发展迅速,正填补从病毒到细胞,甚至组织维度间的结构生物学空白。

2. 新发囊膜病毒的组装及在细胞中复制活动的结构与功能。我们以cryo-ET为主要方法,系统研究多种新发致病型囊膜病毒组装、感染、及免疫的原位结构及机制,最终目的是找到病毒的弱点,并为抗体、疫苗设计提供新思路。


●  代表性论文:


1. Yutong Song#; Hangping Yao#; Nanping Wu#; Jialu Xu#; Zheyuan Zhang#; Cheng Peng; Shibo Li; Weizheng Kong; Yong Chen; Miaojin Zhu; Jiaqi Wang; Danrong Shi; Chongchong Zhao; Xiangyun Lu; Martín Echavarría Galindo; Sai Li; In situ architecture and membrane fusion of SARS-CoV-2 Delta variant, PNAS 2023; 120(18):e2213332120;

2. Dali Wang#; Jiaxuan Li#; Lei Wang; Yipeng Cao; Bo Kang; Xiangfei Meng; Sai Li*; Chen Song*; Toward Atomistic Models of Intact SARS-CoV-2 via Martini Coarse-Grained Molecular Dynamics Simulations, Quantitative Biology 2023; 11(4):421-433;

3. Ye Hong#; Yutong Song#; Zheyuan Zhang; Sai Li; Cryo-electron tomography: the resolution revolution and a surge of in situ virological discoveries, Annual Review of Biophysics, 2023, 52(1):339-360;

4. Sai Li; Cryo-electron tomography of enveloped viruses, Trends in Biochemical Sciences, 2022; 47(2):173-186;

5. Joel D. Allen; Himanshi Chawla; Firdaus Samsudin; Lorena Zuzic; Aishwary Tukaram Shivgan; Yasunori Watanabe; Wan-ting He; Sean Callaghan; Ge Song; Peter Yong; Philip Brouwer; Yutong Song; Yongfei Cai; Helen M. E. Duyvesteyn; Tomas Malinauskas; Joeri Kint; Paco Pino; Maria J. Wurm; Martin Frank; Bing Chen; David I. Stuart; Rogier W. Sanders; Raiees Andrabi; Dennis R. Burton; Sai Li; Peter J. Bond*; Max Crispin*; Site-specific steric control of SARS-CoV-2 spike glycosylation, Biochemistry, 2021, 60(27):2153-2169;

6. Hangping Yao#; Yutong Song#; Yong Chen#; Nanping Wu#; Jialu Xu#; Chujie Sun; Jiaxing Zhang; Tianhao Weng; Zheyuan Zhang; Zhigang Wu; Linfang Cheng; Danrong Shi; Xiangyun Lu; Jianlin Lei; Max Crispin; Yigong Shi; Lanjuan Li*; Sai Li*; Molecular architecture of the SARS-CoV-2 virus, Cell 2020; 183(3):730-738;

ESI hot paper

7. Ying Liu#; Yutong Song#; Siyu Zhang; Min Diao; Shanjin Huang; Sai Li; Xu Tan; PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteins, Cell Discovery, 2020, 6(1):53-67;

8. Xiaoling Wu#; Hua Yue#; Yuanyu Zhang#; Xiaoyong Gao; Xiaoyang Li; Licheng Wang; Yufei Cao; Miao Hou; Haixia An; Lin Zhang*; Sai Li*; Jingyuan Ma; He Lin; Yanan Fu; Hongkai Gu; Wenyong Lou; Wei Wei*; Richard N. Zare; Jun Ge*; Packaging and delivering enzymes by amorphous metal-organic frameworks, Nature Communications, 2019, 10(1):5165;

# Equal contribution; * Co-corresponding

 

●  联系方式:


网页:http://www.lisailab.com

电话:+86-10-62792357

邮箱:sai@mail.tsinghua.edu.cn

通讯地址:球盟会qmh网页版登录生物医学馆A318